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. 2017 Jun 26;12(6):e0177472. doi: 10.1371/journal.pone.0177472

Fig 5. Gal-8 ameliorates EAE and induces Th17 cell death in vitro.

Fig 5

(A) Gal-8 treatment ameliorates MOGp-induced EAE in C57BL/6 mice. The mice were injected daily by intraperitoneal injection of either PBS (Control) or Gal-8 100 μg/ml. Gal-8-treated animals tend to start the disease later and show lower EAE scores during the acute and chronic phases of the disease (*p<0.05; Control, n = 7; Gal-8 treated, n = 5). (B) Gal-8-induced cell death in Th17 lymphocytes differentiated and activated in vitro. Naive (CD62L+ CD44-) CD4+ T cells were purified by cell sorting and differentiated to a Th17 phenotype. The differentiated Th17 cells were isolated with a commercial kit based on the cell surface expression of IL-17 and activated with anti-CD3/anti-CD28 in the presence or absence of Gal-8 (20 μg/ml) for 72 h. Cell death was determined by cell staining with Annexin V and 7-AAD and analyzed by FACS. Representative dot plots show the frequency of Th17 cells before and after purification (upper panels), the selected gate in the forward scatter versus side scatter analysis (middle panels) and the associated contour-plots show the Annexin V versus 7-AAD analysis (lower panels). Numbers in quadrants indicate the percentage of cells in the respective quadrant.