FIG. 6.

Protein-specific IFN-γ production by CD8 T cells using recombinant MVA. Autologous fibroblasts were infected with MVAs expressing one of six different CMV proteins or a control MVA at an MOI of 2:1 for 36 h. Fresh PBMC were added to the infected fibroblasts for 6 h, followed by staining for surface CD8 and cytoplasmic IFN-γ. Representative data for donors 6 (A) and 7 (B) are shown. All values indicate frequencies of CD8 T cells producing IFN-γ in response to the indicated antigen-expressing stimulator cell.