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. 2017 Jun 26;12(6):e0179781. doi: 10.1371/journal.pone.0179781

Fig 1. Vector-borne flaviviruses are specifically restricted in P. leucopus cells in vitro.

Fig 1

(A) Adult skin primary fibroblasts and embryonic fibroblasts from P. leucopus and M. musculus (respectively) were infected with LGTV and assessed by immunofluorescence and probed for E and NS3 proteins. Cell nuclei are stained in blue (DAPI). 20X magnification. P. leucopus and M. musculus fibroblasts were infected with: (B) LGTV (MOI 1 and 10), (C) VSV, (D) POWV-I, (E) POWV-II, (F) TBEV Sofjin, and (G) WNV Kunjin. Supernatants were collected at the indicated time points and quantified by immunofocus or plaque assay. Immunofocus assay data are presented as virus focus-forming units per ml (FFU/ml), while plaque assay data are presented as plaque forming units per ml (PFU/ml). Mean ± SD, data are from three independent experiments performed in triplicate. Asterisks indicate: * = p < 0.05, ** = p <0.01, *** = p <0.0001.