Fig 3. LGTV restriction in P. leucopus cells occurs at the replication phase.

P. leucopus and C57BL/6 cells were infected at MOI 10. RNA lysates were collected at the indicated times post-infection and the resulting cDNA was used as template for RT-qPCR. The quantity of viral RNA was determined by the relative standard curve method to determine the abundance of: (A) LGTV negative strand (-) RNA from cell lysates (B) LGTV positive strand (+) RNA from from virus released into the cell culture supernatants and (C) LGTV focus-forming units (FFU/ml) titrated from virus recovered in the cell culture supernatant. Mean ± SD, data are from three independent experiments performed in triplicate. Asterisks indicate: * = p < 0.05, ** = p <0.01, *** = p <0.0001.