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. 2017 Jun 26;12(6):e0179781. doi: 10.1371/journal.pone.0179781

Fig 4. IFN stimulated proteins are expressed in P. leucopus during LGTV infection.

Fig 4

Expression of ISGs in the reservoir host are shown in (A) Immunoblot of IFIT2 and IFIT3 expressed in P. leucopus and M. musculus cells challenged with mIFN-B, LGTV, VSV or SeV for 8 h or 24 h respectively. (B) Immunoblot of STAT1 expressed in P. leucopus and M. musculus cells challenged with mIFN-β (8 h) and LGTV (24 h and 48 h). Quantitation of STAT1 expression normalized to tubulin and relative to lane 1 is presented below STAT1 panels (C) Quantitation of antiviral assay performed in P. leucopus and M. musculus cells pre-treated with supernatants from corresponding cells that were previously infected with SeV or LGTV. An IFN-β standard curve in the respective species was used to calculate protection from individual samples. Mean ± SD, data are representative from three independent experiments. Hours post infection (hpi), hours post treatment (hpt), no protection (np).