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. 2017 Jun 21;11:71–79. doi: 10.2147/BTT.S134920

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© 2017 Yang et al. This work is published and licensed by Dove Medical Press Limited

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The effect of autophagy inhibitor 3-methyladenine (3-MA) on cervical cancer cells. (A) Acridine orange staining detected autophagic flux in cancer stem cells and adherent cells with and without 3-MA treatment. The orange fluorescence represents autophagosomes. Magnification ×100. (B) xCELLigence RTCA (top) and Transwell (bottom) detected the effects of 3-MA on HeLa cell proliferation and invasion. (C) Western blot detected the effected of 3-MA on the level of PA proteins Oct4, Sox2 and CD133 in both adherent and tumorsphere HeLa cells. ^#P<0.05. Scale bar =100 μm.