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. 2017 Jun 12;13(6):e1006832. doi: 10.1371/journal.pgen.1006832

Fig 2. The LRR-RK MIK2 and CrRLK1L THE1 are major regulators of responses triggered by cellulose biosynthesis inhibition.

Fig 2

(A) Immune marker gene expression in 13-day-old Arabidopsis seedlings determined by qRT-PCR. Seedlings were mock treated, or treated with 0.6 μM ISX, 6 μM DCB, or 0.4 μM TXT for 9 h. Expression of the immune marker genes FRK1, At1g51890, and CYP81F2 was normalized relative to U-box expression values. Depicted is the fold change in expression relative to mock treatment. Error bars represent standard error of three technical replicas. (B-E) JA (B) and SA production (C) and lignin-deposition (D,E) in 6-day-old Arabidopsis seedlings, mock treated or treated with 0.6 μM ISX for 7 h (B,C) and 12 h (D,E). Error bars represent standard error of n = 4 (B,C) or n = 20 (E) biological replicas. (B) The upper and lower panel display the same data, yet in the lower panel, the y-axis has been adjusted to visualize the JA levels in mock-treated samples. (D) The size bar represents 100 μm. (A-E) Asterisks indicate a statistically significant difference relative to Col-0, as determined by a two-tailed Student’s T-test (p < 0.05). Experiments were repeated at least three times with similar results.