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Nuclease-resistant DNA in transfected cell lysates. Cells transfected with L1 alone or cotransfected with L1 and L2 were lysed in the presence of a nuclease cocktail. DNA was then extracted from the lysate, separated on an agarose gel, and stained with SYBR Green I. (A) Untreated nuclease-resistant DNA samples; (B) the same samples digested with BglII. Purified target plasmid control is shown in the far right lanes.
