FIG. 7.

(A to C) Western blots of cells transfected with cysteine mutant L1 constructs. Cells were cotransfected with a construct expressing HPV16 L2 together with wild-type or cysteine mutant L1 constructs C175S or C428S. The cells were lysed and then incubated at 37°C for 10 min or 18 h. The chart at the bottom of the figure shows the titer of the lysates standardized to the titer found in the wild-type L1 lysate after 10 min of incubation at 37°C.