Fig 2. Sch9 does not impact on vesicular trafficking.

(A-E) Sorting and processing of vacuolar proteases is not impaired in exponentially growing sch9Δ cells. Processing of CPY (A), ALP (C) and Ape1 (E) was examined by Western blot. * represents cross-reacting band. Intracellular localization of CPY-GFP (B) and GFP-Pho8 (D) was examined by fluorescence microscopy. (F) Sch9 affects basal and non-specific autophagy. Exponentially growing cells expressing Pho8Δ60 were shifted to nitrogen starvation medium. Samples were taken at the indicated time points, proteins extracted, and specific activities determined. Results are normalized to the activity of a WT strain starved for 24h. Mean values ± SD are shown (unpaired t-test). See also S1 and S2 Figs.