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. 2017 Jun 12;13(6):e1006835. doi: 10.1371/journal.pgen.1006835

Fig 4. Effects on colony size and growth by deletion of VPH1, STV1 and/or SCH9.

Fig 4

(A, B) A synthetic sick phenotype arises when deletion of SCH9 is combined with a fully dysfunctional V-ATPase. (A) Tetrad dissection of the diploid strain JW 04 952 (sch9Δ/SCH9 vph1Δ/VPH1 stv1Δ/STV1). (B) Colony sizes were calculated, normalized relative to WT and are shown as mean values ± SD. Letters indicate groups of strains with a significant difference in colony size (p < 0.001, one-way ANOVA). (C, D) Strains combining deletion of SCH9 with a fully dysfunctional V-ATPase show a deteriorated growth phenotype. OD600nm was followed over time in fully supplemented medium without buffer (C) or buffered at pH 5 (D). A representative experiment with at least 4 independent colonies for each strain is shown. Error bars represent SD from the mean. See also S4 and S5 Figs.