Skip to main content
. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Radiat Res. 2016 Nov 21;186(6):592–601. doi: 10.1667/RR14423.1

FIG. 1.

FIG. 1

Panels A and B: Drawings of the on-line SARRP BLT and off-line BLT/CBCT systems, respectively. Both systems adopt the similar configuration of the CBCT and optical assembly. The optics assembly contains the 3-mirror system, filter wheel and CCD camera. CBCT is acquired with X-ray source and detector panel in the horizontal setup. The 3-mirror system reflects the bioluminescence emitted from the mouse surface to the CCD camera via the optical path (red arrow). For the off-line system, all the components are enclosed in lead shielding. Panel C: Actual on-line SARRP BLT system. Panel E: Imaging chamber of the off-line system. Panel D: Portable mouse bed used for animal transport between the on-line and off-line systems. A gas tube was connected to the isoflurane/oxygen flow to keep the mouse anesthetized during transport and imaging procedures. In accordance with NIH requirements, a mouse phantom instead of a real animal is shown (panels C and E inserts, panel D) to illustrate the animal being imaged and transported between the two systems. In the real animal experiments, the mouse was taped for immobilization and eight lead pellets were used as imaging markers.