Figure 5.
Effect of treatment with α-bis-LNCs on MAPK pathway activation.
Notes: The lungs were perfused and collected to analyze protein expression 18 hours after LPS challenge and the pretreatment with α-bis, α-bis-LNCs, or ULNCs at a dose of 50 mg kg−1. Representative Western blotting analysis of the phosphorylation state of (A) p38, (B) ERK1/2, and (C) JNK. The densitometric values obtained for phosphorylated and total protein from treatments were normalized to their respective controls that were not exposed to LPS (control bar; 100%). Data are expressed as a ratio of the normalized percentages of phosphorylated and total protein. Bars represent the mean ± SEM (n=5–7). +P<0.05 compared with the saline group; *P<0.05, **P<0.01, and ***P<0.001 compared with the LPS-induced group.
Abbreviations: α-bis, α-bisabolol; α-bis-LNCs, α-bisabolol-loaded lipid-core nanocapsules; LNCs, lipid-core nanocapsules; ULNCs, drug-unloaded nanocapsules; LPS, lipopolysaccharide; MAPK, mitogen-activated protein kinase; SEM, standard error of mean.