Table 1. Primers sequences for gene expression.
| Gene | Forward (5’-->3’) | Reverse (5’-->3’) |
|---|---|---|
| Gapdh | TGAAGCAGGCATCTGAGGG | CGAAGGTGGAAGAGTGGGAG |
| Hamp | CCTGAGCAGCACCACCTATCT | GCTTTCTTCCCCGTGCAAAGG |
| Slc11a2 | TTGGCAATCATTGGTTCTGA | CTTCCGCAAGCCATATTTGT |
| Slc40a1 | ATGGGAACTGTGGCCTTCAC | TCCAGGCATGAATACGGAGA |
| Vegf | CCACGTCAGAGAGCAACATCA | TCATTCTCTCTATGTGCTGGCTTT |
| Il10 | GCTCTTACTGACTGGCATGAG | CGCAGCTCTAGGAGCATGTG |
| Dcytb | CATCCTCGCCATCATCTC | GGCATTGCCTCCATTTAGCTG |
| TNFa | AGGGTCTGGGCCATAGAACT | CCACCACGCTCTTCTGTCTAC |
| Slc11a2-IRE | TGTTTGATTGCATTGGGTCTG | CGCTCAGCAGGACTTTCGAG |
| TfR1 | GGAAGACTCTGCTTTGCAGCTAT | GCCCAGGTAGCCCATCATGA |
| Hif-2a | TGAGTTGGCTCATGAGTTGC | TATGTGTCCGAAGGAAGCTG |
| Ccl2 | ATTGGGATCATCTTGCTGGT | CCTGCTGTTCACAGTTGCC |
| Cxcl1 | TCTCCGTTACTTGGGGACAC | CCACACTCAAGAATGGTCGC |
| Adipoq | GGAGATGCAGGTCTTCTTGG | ATGTTGCAGTAGAACTTGCC |
| Lep | TGAAGCCCAGGAATGAAGTC | TCAAGACCATTGTCACCAGG |
Abbreviations: Hamp, hepcidin; Slc11a2, DMT1; Slc40a1, ferroportin; Vegf, Vascular endothelial growth factor; Il10, interlukin 10; Dcytb, Duodenum cytochome B; TNFα tumor necrosis factorα; TfR1, transferrin receptor 1; HIF-2α, Hypoxia inducible factor 2α; Adipoq, adiponection; Lep, leptin.