FIG. 5.

Comparative proliferative responses of 7/16-5 T cells derived from TCR single-Tg and 7/16-5 × HBe(hi) double-Tg mice. Unprimed spleen cells from 7/16-5 single-Tg or 7/16-5 × HBe(hi) double-Tg mice were labeled with the intracellular dye CFSE (0.5 mM) and cultured with 0.5 μg of the HBc/HBeAg-specific p120-140 for 4 or 7 days. Cultured cells were harvested at 4 or 7 days and labeled with anti-TCR Vβ11 antibodies. The number of cell divisions is measured by the dilution of the CFSE dye, which is reduced by 50% at each cell division, in TCR Vβ11⁺-gated T cells by flow cytometry. The numbers above the windows represent the percentage of Vβ11⁺ T cells that have undergone the indicated number of cell divisions. The data are representative of the results from two separate experiments.