Figure 4. Injecting acid into the amygdala before retrieval enhances exchange of AMPARs.
(A) Schematic of experimental procedure. Aversive conditioning was done as in Figure 1A. On day 2, mice received a microinjection of acidic saline (to reduce pH to ~6.8) or saline (pH ~7.35) into the amygdala. Five minutes later, they were presented with a single tone or not. Ten minutes after that, brain slices were prepared and AMPAR-EPSCs were recorded. (B) AMPAR current-voltage relationships in lateral amygdala pyramidal neurons. (C) Rectification index of AMPA-EPSCs. Data are mean±SEM. n = 18–24 for each group. * indicates p<0.05 by ANOVA with Tukey’s post hoc multiple comparison. No ret + saline vs Ret + saline, p=0.0431; No ret vs No ret + acidic saline, p=0.9781; No ret vs Ret + acidic saline, p<0.0001; No ret (WT) vs Ret + acidic saline (Asic1a−/−), p=0.9851; Ret + saline vs No ret + acidic saline, p=0.6081; Ret + saline vs Ret + acidic saline, p=0.0002; Ret + saline (WT) vs Ret + acidic saline (Asic1a−/−), p=0.5596; No ret + acidic saline vs Ret + acidic saline, p<0.0001; No ret + acidic saline (WT) vs Ret + acidic saline (Asic1a−/−), p>0.9999; Ret + acidic saline (WT) vs Ret + acidic saline (Asic1a−/−), p<0.0001.