(a) Spontaneous activity during days 1–10 and 10–20 of adulthood for N2 grown on Empty Vector RNAi (n = 80), N2 grown on odr-10 RNAi (n = 40), daf-2 grown on Empty Vector RNAi (n = 40), and daf-2 grown on odr-10 RNAi (n = 39). *p<0.05; **p<0.01; ***p<0.001. (b) Stimulated activity for the same individuals shown in panel (a). (c) Response duration for the same individuals shown in panel (a). (d) Response latency for the same individuals shown in panel (a). (e) Spontaneous activity during days 1–10 and 10–20 of adulthood for N2 grown on Empty Vector RNAi (n = 80), N2 grown on daf-2 RNAi (n = 38), odr-10(ky32) mutants grown on Empty Vector RNAi (n = 40), and odr-10(ky32) mutants grown on daf-2 RNAi (n = 38). *p<0.05; **p<0.01; ***p<0.001. (f) Stimulated activity for the same individuals shown in panel (e). (g) Response duration for the same individuals shown in panel (e). (h) Response latency for the same individuals shown in panel (e). (i–l) Survival curves.
DOI:
http://dx.doi.org/10.7554/eLife.26652.025
Figure 8—source data 1. Includes data for lifespan, spontaneous locomotion, stimulated locomotion, response duration, and response latency for each strain shown.