FIG. 6.

LF variants and inhibition of transmission. (A) DC were preincubated with bLF, the C-lobe fragment of bLF, hLF, apo-hLF, or holo-hLF. After HIV-1 incubation and washing, DC were cocultured with LuSIV cells. The results of eight independent experiments are combined; transmission without inhibitor was set at 100%. Error bars indicate standard deviations. (B) Binding of LF variants to DC-SIGN. Several amounts of the LF variants were applied to an ELISA plate, and the adhesion of soluble DC-SIGN-Fc was measured by anti-IgG1 ELISA. At the highest concentration of LF, DC-SIGN-blocking antibody AZN-D1 was also added. As a positive control, we applied gp120. OD 450, optical density at 450 nm; n.d., not done.