Table 2.
Gene-specific primers of the lipid metabolism related enzyme
| Gene name | Primer sequence (5 → 3) | Tm (°C)/size (bp) | Accession |
|---|---|---|---|
| ACC | F:AATGGCAGCTTTGGAGGTGT R:TCTGTTTGGGTGGGAGGTG |
60.9/136 | NM205505 |
| FAS | F:CTATCGACACAGCCTGCTCCT R:CAGAATGTTGACCCCTCCTACC |
62.0/107 | J03860 |
| SCD1 | F:ACCATACATTCCCCTACGACT R:TTTTCCGGGCCAAGATGACC |
56.0/144 | NM204890 |
| SREBP-1c | F:GAGGAAGGCCATCGAGTACA R:GGAAGACAAAGGCACAGAGG |
60.3/220 | AY029224 |
| PPARα | F:TGGACGAATGCCAAGGTC R:GATTTCCTGCAGTAAAGGGTG |
60.3/813 | AF163809 |
| CPT-1 | F:CAATGAGGTACTCCCTGAAA R:CATTATTGGTCCACGCCCTC |
57.5/337 | AY675193 |
| ACOX | F:ATGTCACGTTCACCCCATCC R:AGGTAGGAGACCATGCCAGT |
54.0/133 | NM001006205 |
| PPARγ | F:CCAGCGACATCGACCAGTT R:GGTGATTTGTCTGTCGTCTTTCC |
57.5/145 | AF163811 |
| ATGL | F:TCCTTCACCTTCAGCGTCCA R:AGTGTTGTCCTCCATCTGGTC |
54.0/113 | EU852334 |
| LPL | F:CAGTGCAACTTCAACCATACCA R:AACCAGCCAGTCCACAACAA |
60.0/150 | NM205282 |
| GADPH | F:GGTGAAAGTCGGAGTCAACGG R:CGATGAAGGGATCATTGATGGC |
58.4/108 | NM204305 |
ACC acetyl-CoA carboxylase, FAS fatty acid synthase, SCD1 stearoyl-CoA desaturase-1, SREBP-1c sterol regulatory element binding protein-1c, PPARα peroxisome proliferator activated receptor α, CPT-1 carnitine palmitoyltransferase-1, ACOX acyl CoA oxidase, PPARγ peroxisome proliferator activated receptor γ, ATGL adipose triglyceride lipase, LPL lipoprotein lipase, GADPH glyceraldehyde-3-phosphate dehydrogenase