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. 2017 Jun 26;7:4221. doi: 10.1038/s41598-017-04236-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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pH-dependent activity profile of UmpAB. The antiport activity was measured by the fluorescence dequenching method. Na⁺/H⁺ antiport activity (filled circle), Li⁺/H⁺ antiport activity (filled square) and K⁺/H⁺ antiport activity (filled triangle) were measured at the indicated pH values. The wavelength of excitation light was 492 nm and fluorescence was monitored at 526 nm. Each value point represents the average of three independent determinations.