FIG. 2.

Growth of HAV in cell lines transfected with virion RNA. Monolayers of MMH-D3, Ltk-, and GL37 cells grown in 25-cm² flasks were transfected with virion RNA. At 6 h posttransfection, cells were washed three times, split 1:3, and grown at 35°C in a CO₂ incubator. Cells were split 1:3 weekly; one-third of the collected cells were subjected to three freeze-thaw cycles, cell debris was pelleted, and the supernatant containing HAV was stored at −70°C. HAV was titrated as described in the legend to Fig. 1. Error bars, standard deviations.