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. 2017 Jun 26;7:4259. doi: 10.1038/s41598-017-04245-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The alignment of consensus LSU rDNA sequences of 21 Pseudo-nitzschia species. The sequences were aligned using the Bioedit Sequence Alignment v. 7.0.5.3. The common forward and reverse primers flanked species-specific regions of the 21 Pseudo-nitzschia species considered under investigation. The target LSU rDNA amplified region was 130–133 bp in length and located in the 384 to 514 nucleotide position.