Table 2.
Primers and joint DNAs used in this study.
| Primer | Sequences 5′-3′ | Use | Reference |
|---|---|---|---|
| SIX3-F3 | TGTCCATGACATGGGTTTGC | SIX3 detection | This study |
| SIX3-B3 | CAGTCCCGAATTGCCAGC | This study | |
| SIX3-FIP | TATGGGTTACCCCGTCCTTGGA-TGGGCTACCTGTGGAAGA | This study | |
| SIX3-BIP | ACGAGCTTCAGCACCGAACC-CGCTCATATGGAGGGTCTCT | This study | |
| SIX3-LF | GCTGACCGACAGATGAATCGG | This study | |
| SIX3-LB | TCTGGGTTATGCTAGAATGTTACAC | This study | |
| Joint DNA 1 | TGCGTGTTTCCCGGCC - UQprobe* | This study | |
| Joint DNA 2 | CCCGGCCGCCGCACGTCTTCTAC - UQprobe* | This study | |
| SIX3 F | TATATTACCGACCATCTTGCCTAAACATTTACCA | SIX3 mutagenesis | This study |
| SIX3 R | GCCAAGGGGAACTGCCACAG | This study | |
| G137CF | TGCGTGTTTCCCGGCCGCCCCACGTCTTCTACTTCATTTACTA | This study | |
| G137CR | AAATGAAGTAGAAGACGTGGGGCGGCCGGGAAACACGCAAT | This study | |
| SIX4 F | ACTCGTTGTTATTGCTTCGG | SIX4 detection | Inami et al.11 |
| SIX4 R | CGGAGTGAAGAAGAAGCTAA | Inami et al.11 | |
| SIX4-F3 | TCCAGTTGGAGCAAGTTGG | Ayukawa et al.18 | |
| SIX4-B3 | TGCCCGTCTCTGCGATAG | Ayukawa et al.18 | |
| SIX4-FIP | GCCTCCTTGTCATCTACCGCATTTGGGGTGATAGAGAGCCTG | Ayukawa et al.18 | |
| SIX4-BIP | ATGGCAAAGTTGTCACGCGTGTTGGGCCTTGAGTCGAATG | Ayukawa et al.18 | |
| SIX4-LF | GACGGAGAGCAAGTAGCGT | Ayukawa et al.18 | |
| SIX4-LB | CAGGAAAGCCAGGAATAGGACGA | Ayukawa et al.18 |
*3′ ends of joint DNAs consist of UQprobe-G complementary sequences