Table 3.
Comparison of molecular detection for Fol race 3 from field soil of Kochi prefecture.
| Sample | Sampled date | Symptom | Location | LAMP with the universal QProbe | LAMP | PCR | ||
|---|---|---|---|---|---|---|---|---|
| Melting temperature (arithmetic mean °C ± standard deviation) | ||||||||
| Joint DNA 1 | Joint DNA 2 | SIX4 | SIX3 | SIX4 | ||||
| DNA of KoChi-1 | — | — | — | 53.5 ± 0.2 | 70.1 ± 0.3 | + | + | + |
| Soil DNA 1 | 2012.5 | Yes | N33°31′92″ E133°21′97.1″ | 53.4 ± 0.4 | 70.0 ± 0.1 | + | — | + |
| Soil DNA 2 | 2013.5 | Yes | N33°31′50.28″ E133°22.01′66″ | 53.8 ± 0.2 | 69.7 ± 0.4 | + | — | — |
| Soil DNA 3 | 2012.11 | No | N33°31′92″ E133°21′97.1″ | — | — | — | — | — |
| Soil DNA 4 | 2014.11 | No | N33°31′50.28″ E133°22.01′66″ | — | — | +a | — | — |
+, detection −, no detection a, LAMP products were obtained only once in three replicates.