Figure 5.

IL-33 enhances the accumulation of pulmonary ILC2s in OVA-sensitized mice. Flow cytometric analysis of lung cells. (a) ILC2s were identified as SSC^lowFSC^lowCD45⁺Lin⁻ICOS⁺KLRG1⁺Sca-1⁺CD25⁺ ST2⁺ cells. Gates were set based on FMO controls, FMO ctrl = overlay of the two FMO controls for each respective plot. The representative FMO plots and plots showing the representative SSC^lowFSC^lowCD45⁺ gating belong to the OVA + IL-33 group. (b) Frequency and numbers of pulmonary ILC2s. (c) Quantification of inflammatory lung cells. Alveolar macrophages were identified as CD45⁺ SiglecF⁺ CD11c⁺, eosinophils as CD45⁺SiglecF⁺CD11c⁻CD11b⁺Gr-1^lo, neutrophils as CD45⁺ SiglecF⁻ CD11c⁻Gr-1^hiCD11b^hi and CD4+ cells as CD45⁺CD11b⁻ CD4⁺ (frequency and representative plots including FMO controls can be found in Supplementary Fig. S1). *p < 0.05, **p < 0.01, ***p < 0.001 (ANOVA, Bonferroni). Results are pooled data of four independent experiments (mean ± SEM of n = 8 mice in each group).