Skip to main content
. 2017 Jun 26;7:4230. doi: 10.1038/s41598-017-04275-7

Figure 2.

Figure 2

Synergistic effect between huLTF and LTF-Abs. (A) Purified human monocytes (Mo, upper panels) and differentiated macrophages (MФ, lower panels) were stained with FITC-conjugated mAbs against human CD14 or CD11b followed by FACS analysis. (B) Freshly fractionated human monocytes and (C) human monocytes-differentiated macrophages were incubated with 15 μg/ml huLTF, or RA-IgG, or RA-IgG-IC (mixture of huLTF plus RA-IgG), or huLTF plus IVIG (15 μg/ml for each protein), for 24 h. (D) Fractionated human monocytes and (E) human monocytes-differentiated macrophages were incubated with 15 μg/ml huLTF, or mouse anti-huLTF mAb M860, or rabbit anti-huLTF polyclonal IgG L3262, or a mixture of huLTF plus M860 (LTF + M860) or L3262 (LTF + L3262) for 24 h. Cells cultured in medium only (Med) or stimulated with 3 μg/ml LPS were included as controls. TNF-α concentration in the culture supernatant was then determined by ELISA. Data are mean concentration ± SEM from triplicate wells and these are representatives of at least 3 independent experiments. *p < 0.05, **p < 0.01 compared with medium controls.