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. 2005 Mar;79(5):2859–2868. doi: 10.1128/JVI.79.5.2859-2868.2005

FIG. 4.

FIG. 4.

Measurement of EGFP mRNA in LMH/2A and DF-1 cells transfected with the positive-control CMV promoter vector pEGFP-N1 or one of the CAV promoter constructs, pEGFP-S5, pEGFP-L5, pEGFP-SE, or pEGFP-LE. The number of DNA copies transfected into the cells and the mRNA transcripts were measured by qPCR or qRT-PCR. (A and B) LMH/2A (A) and DF-1 (B) transfected with pEGFP-N1, pEGFP-S5, or pEGFP-L5. Results are from one experiment with five separate transfected wells. (C) LMH/2A cells transfected with pEGFP-N1, pEGFP-SE, or pEGFP-LE from one assay, with no analysis of significance due to only one sample per treatment. (D) DF-1 cells transfected with pEGFP-N1, pEGFP-SE, or pEGFP-LE from two separate experiments with one to four separate wells per experiment. Results are reported as mRNA copy number and are normalized for transfection efficiency by dividing by DNA copies of the expression plasmid. RNA and DNA EGFP copies were normalized to the cellular genes GAPDH and iNOS, respectively. Columns within a panel with different letters are significantly different, with a P value of <0.05.