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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Stem Cells. 2017 Apr 24;35(7):1860–1865. doi: 10.1002/stem.2628

Figure 3.

Figure 3

Effects of Slit2 stimulation on hfNPCs migration. A and B, Schematic representation of a transwell migration and chemotaxis assay, respectively. C–D, Quantification of transwell migrated cells shows a reduced number of cells when Slit2 (200ng/ml) was present in the bottom well using two different primary cell cultures. E, Basal cell migration in a 2D chemotaxis assay shows aleatory migration distribution. F, hfNPCs show a biased cell migration pattern in the presence of a Slit2 gradient, indicating a chemorepellent response. G, hfNPC migration persistence is increased in response to a gradient of Slit2. H, The active forms of the small Rho-GTPases CDC42 and Rac1 are decreased upon Slit2 stimulation. **p<0.01, *p<0.05.