FIG. 4.

Schematic representation of the gH deletion and mutation constructs. The top line shows a wt-gH linear map with predicted α-helix and TM regions. The second line shows the sequence of the α-helix, residues 378 to 397, and flanking residues. In gH_Δ378-397 the α-helix was collapsed, after insertion of two SphI sites at residues 377 and 397. In gH_Δ378-387 residues 378 to 387 were collapsed after insertion of SphI sites at residues 377 and 387. gHσ_Leu carries the L382P, GLL384-386WPP substitutions.