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. 2017 Jan 19;28(2):274–283. doi: 10.1111/bpa.12482

Figure 3.

Figure 3

Biochemical analysis of tau in Triton X‐100 insoluble fraction extracted from frozen brain tissues. (A) Total‐tau (17025) and (B) phosphorylated‐tau (PHF‐1) immunoblot showed abundance of tau pathology in case 1 and case 2 Hp but not in case 1 Pu. Isoform‐specific immunoblot revealed predominant 4R‐tau pathology in both cases (C) consistent with IHC finding. Furthermore, case 1 Hp was also immune‐reactive to 3R‐tau antibody (d). Hp = hippocampus, Pu = putamen; Alzheimer disease (AD), corticobasal degeneration (CBD) and Pick's disease (PiD) control samples are also shown.