TABLE 1.
Genes regulated in vaginal tissue of mice infected with HSV-2 under the influence of progesteronea
| Gene class | Gene | Description | Regulation |
|---|---|---|---|
| Small inducible cytokines | Scya5 (RANTES) | Chemokine (C-C motif) ligand 5 | ↑ |
| Scya6 (C10) | Chemokine (C-C motif) ligand 6 | ↑ | |
| Scya7 (MCP-3, MARC) | Chemokine (C-C motif) ligand 7 | ↑ | |
| Scya21a (6Ckine/SLC/exodus-2) | Chemokine (C-C motif) ligand 21a (serine) | ↑ | |
| Scyb2 (MIP-2) | Chemokine (C-X-C) ligand 2 | ↑ | |
| Scyb5 (LIX/GCP-2/ENA78) | Chemokine (C-X-C) ligand 5 | ↑ | |
| Scyb10 (IP-10) | Chemokine (C-X-C) ligand 10 | ↑ | |
| Scyb11 (eotaxin) | Chemokine (C-X-C) ligand 11 | ↑ | |
| Scyb15 | Chemokine (C-X-C) ligand 15 | ↑ | |
| Chemokine receptors | Cmkbr7 (CCR-7) | Chemokine (C-C motif) receptor 7 | ↑ |
| Ltb4r2 | Leukotriene B4 receptor 2 | ↑ | |
| Tapbp | TAP binding protein | ↑ | |
| Other related genes | SDF-1a | Chemokine (C-X-C motif) ligand 12 | ↓ |
| Epo | Erythropoietin | ↑ |
Chemokine and chemokine receptor genes were analyzed by microgene array analysis. OVX mice were treated with E2 or P4 and inoculated IVAG with 105 PFU of HSV-2 or left untreated. Three days later, total RNA was extracted from vaginal tissue and reverse transcribed to cDNA and labeled with [32P]dCTP. Labeled cDNA was hybridized to GE Array membranes, and signals were analyzed with a phosphorimager, as described in Materials and Methods. The signals of the chemokine and receptor genes were normalized to a housekeeping gene (GAPDH) and expressed as the percent GAPDH. ↑, upregulation; ↓, downregulation. Any genes that were expressed ≤20% GAPDH were not included in the analysis. The data summarized in this table is from two separate experiments.