Table 2.

Cytokine production by splenocytes.

	Control	Maternal LPS
IFN-γ (ng/ml)	3.51 ± 0.234	4.69 ± 0.324*
IL-2 (ng/ml)	1.39 ± 0.233	1.66 ± 0.325
IL-4 (pg/ml)	49.12 ± 3.353	47.88 ± 2.534
IL-6 (pg/ml)	45.70 ± 4.500	58.80 ± 4.700
IL-10 (pg/ml)	175.63 ± 49.05	295.02 ± 35.634*
IL-17 (ng/ml)	0.69 ± 0.070	0.64 ± 0.082
TNF-α (pg/ml)	214.70 ± 6.185	142.94 ± 26.727*
IFN-γ/IL-4 ratio	73.00 ± 9.330	102.61 ± 9.792*

Splenocytes (5 × 10⁵cells/100 μl per well) were cultured after 48 h of culture in the presence of 100 μg/ml ovalbmin(OVA), 5 μg/ml concanavalin A (ConA), or 10 μg/ml lipopolysaccharide (LPS) as a non-specific response at 37 °C in 5% CO₂. ELISA was used on supernatans of splenocytes stimulated with either LPS(IL-6,TNF-α) or ConA(others). Values are mean ± SE, *; P < 0.05