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. 2017 Jun 7;13:415–431. doi: 10.1016/j.dib.2017.06.001

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 2 — Proportions and characteristics of PDGFRα^GFP cells obtained by direct FACS compared to isolation by differential adherence. Flow cytometry profile of lung cell lineage proportions and PDGFRα^GFP proportions of fresh whole cell suspension or after selection by differential adherence. Single cell suspensions were stained and subjected to FACS directly after isolation (whole prep) or following incubation for 2 h to obtain non-adherent cells (supernatant) and adherent stromal cells (adherent). (A) Relative proportion of hematopoietic (CD45⁺), epithelial (CD326⁺), endothelial (CD31⁺), CD140a⁺ (PDGFRα⁺) stromal, and CD140a^neg stromal cell populations analyzed by FACS in samples obtained from whole-lung suspension vs. non-adherent and adherent cells following differential adherence. (B) Distribution of PDGFRα-GFP^bright and PDGFRα-GFP^dim stromal cells contained in non-adherent and adherent cell fractions following differential adherence. (C) Proportion of PDGFRα-GFP^bright (dark green) and PDGFRα-GFP^dim (light green) fibroblasts contained in single cell suspensions by direct FACS or FACS following differential adherence. (D) The relative proportion CD29⁺, CD34⁺CD29⁺, CD34⁺ or CD29^-CD34^- subpopulations in CD140a⁺ cells analyzed by direct FACS or FACS following differential adherence.