Figure 4.

Glucocorticoid regulation of lipolysis in vitro. Data are given as mean ± SEM for glycerol release from paired primary human subcutaneous (A) and visceral adipocytes (B) (both n=11) cultured in 0 nM (white columns), 100 nM (grey columns) or 1000 nM cortisol (black columns) for 24 hours in the presence of either vehicle, 100 pM insulin or 10 µM adrenaline. Insulin suppressed and adrenaline increased glycerol release. Cortisol did not alter glycerol release from adipocytes in vehicle treated cells, but increased glycerol release in subcutaneous adipocytes co‐incubated with insulin. Cortisol decreased glycerol release in visceral adipocytes co‐incubated with adrenaline. *P < .05 vs 0 nM; $P < .05, $$$P < .001 vs vehicle 0 nM