Figure 6.

BldO has a single target, whiB.

A. Genome‐wide distribution of BldO binding sites identified by ChIP‐seq analysis using M2 anti‐FLAG antibody, conducted at the onset of sporulation on the bldO null mutant complemented in trans with a functional bldO‐3xFLAG allele expressed under the control of the native promoter from the ΦBT1 integration site. Strains were grown in DNB medium. The peak upstream of whiB is marked by the red arrow. Peaks also seen in the negative control (wild‐type S. venezuelae), such as those indicated by asterisks, were excluded from further analysis.

B. Close‐up of a ∼5‐kb region around whiB. Colour‐coding of the ChIP samples is as follows: the onset of sporulation (bldO‐3xFLAG, green), S. venezuelae wild‐type anti‐FLAG negative control (WT, purple). Genes running left to right are shown in green, and genes running right to left are shown in red. The black arrow indicates the gene (whiB) subject to BldO regulation.