Figure 7.

BldO binds to a sequence overlapping the transcriptional start site and the −10 region of the developmentally induced whiBp2 promoter.

A. DNase I footprinting analysis of BldO bound to radiolabelled probes derived from the (forward) and (reverse) sequence upstream of whiB. 5′ end‐labelled probes were incubated with increasing concentrations of BldO (indicated in µM above the lanes) and subjected to DNase I footprinting analysis. The footprints are flanked by Maxam and Gilbert sequence ladders (GA) and the black brackets indicate the positions of the BldO‐protected regions. Asterisks mark phosphodiester bonds that are not protected from cleavage.

B. Summary of the DNase I footprinting results, showing the protected sequences on the forward and reverse strands (black brackets) relative to the transcriptional start site (+1) and the −10 and −35 sequences (underlined). The sequence of the protected region is also shown above with the hyphenated inverted repeat highlighted in red. Asterisks mark phosphodiester bonds that are not protected from cleavage.