Figure 2.

Inflammation accompanied by DNA damage emerges in HOIP‐deleted livers. (A) Hepatic CD45⁺ cell count per gram of Hoip^flox (open circles) and Hoip^Δhep mice (closed circles) at the indicated ages. (B) Relative quantification of the transcript levels of Tnf, Ccl3, and (Cxcl1) in Hoip^flox and Hoip^Δhep livers (n = 4 each). Levels of transcripts were normalized to those of Hoip^flox livers. (C) Liver cells from Hoip^flox and Hoip^Δhep mice at 4 weeks of age were stained, and the numbers of hepatic immune subpopulations were quantified by flow cytometry. (D) Ki‐67 staining (red) of liver sections from Hoip^flox and Hoip^Δhep mice at 4 weeks of age. The dot plot shows the quantification of Ki‐67⁺ cells per optical area. (E) γH2AX staining (red) of liver sections from Hoip^flox and Hoip^Δhep mice at 4 weeks of age. The dot plots show the quantification of γH2AX⁺ cells per optical area. Unpaired two‐tailed t test was employed for statistical analysis. Abbreviations: Bc, B220⁺ cells; CD4 Tc, CD3⁺ CD4⁺ cells; CD8 Tc, CD3⁺ CD8⁺ cells; Gr, Ly6G⁺ cells; Mo/MF, F4/80⁺ cells; NK, CD3⁻ NK1.1⁺ natural killer cells; NKT, CD3⁺ NK1.1⁺ natural killer T cells.