Figure 3.

HOIP deletion in the liver parenchyma results in increased apoptosis. (A) Levels of serum ALT in Hoip^flox (open circles) and Hoip^Δhep mice (closed circles) at the indicated ages. (B) Hematoxylin and eosin staining of the livers of Hoip^flox and Hoip^Δhep mice at the indicated ages. Black arrowheads indicate apoptotic bodies, and white arrowheads indicate areas showing the ductular reaction. (C) Quantification of CC3⁺ and TUNEL⁺ cells per field of Hoip^flox and Hoip^Δhep liver sections. (D) Lysates of primary hepatocytes from Hoip^flox and Hoip^Δhep mice at 8‐10 weeks of age were immunoblotted for CC8 and CC3. Unpaired two‐tailed t test was employed for statistical analysis. Abbreviation: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase.