Figure 6.

Hepatocyte apoptosis in HOIP‐deficient livers is unaffected by genetic ablation of TNFR1. (A) Levels of serum ALT in Hoip^flox, Hoip^Δhep, and Tnfr1^KO Hoip^Δhep mice at the indicated ages. (B) Representative pictures of hematoxylin and eosin sections of the livers of Tnfr1^KO Hoip^Δhep mice at 2 and 4 weeks of age. Arrowheads indicate apoptotic bodies. (C) Quantification of CC3⁺ and TUNEL⁺ cells per area of the livers of Hoip^flox, Hoip^Δhep, and Tnfr1^KO Hoip^Δhep mice at 2 weeks of age. (D) Lysates of primary hepatocytes from Hoip^flox (F), Hoip^Δhep (Δ), and Tnfr1^KO Hoip^Δhep (TΔ) mice at 8 weeks of age were immunoblotted for CC8 and CC3. (E) Immunoprecipitation of the FADD‐associated complex in zVAD‐treated (24 hours) primary hepatocytes from Hoip^flox (F), Hoip^Δhep (Δ), and Tnfr1^KO Hoip^Δhep (TΔ) mice. Asterisks indicate unspecific bands. Unpaired two‐tailed t test was employed for statistical analysis. Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; IP, immunoprecipitation.