Figure 2.

CHO GAA producing and control cell line comparison.

(A): cell growth performance; (B): product production via western blot; (C): product production quantification via GAA diagnostic assay.21 Although cell line 2 was the highest producer, cell line 1 was the fastest grower. Cell line 1 was chosen due of good balance between growth and production performance. Data show that null (N) cell line produces non detectable product as expected, and pool sample (P) secretes average level of GAA. Pool sample is the sample used for cell line selection (all cell lines mixed). Medium used ThermoFisher scientific CD‐CHO with 250 µg/mL HygB, viability >93% at day 6 (null grown in same media without HygB).