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. 2017 Mar 29;33(3):666–676. doi: 10.1002/btpr.2455

Figure 4.

Figure 4

Elution peak in AIEX chromatogram (A) is divided in four fractions and analyzed. Western blot analysis (B) shows absence of GAA in flowthrough and strip, and its presence in the four elution fractions, with the main amount eluting in fraction 2 and 3 (prestained molecular weight standard cat# LC5925 Thermo Fisher). For SDS‐PAGE SYPRO ruby (C) fractions were combined (1–2 and 3–4). These data show presence of GAA at around 110KDa in both fractions together with lower molecular species in minor quantity (ladder molecular weight standard cat# 26614 Thermo Fisher). Clarified Harvest (H) was also run along for comparison.