Table 1.
Overlapping and Unique HCPs
| Name (μg/ml) | GAA | null | Ab |
|---|---|---|---|
| Clusterin | 3.5 | 7.6 | 0.2 |
| Complement C3 | 3.4 | 13.5 | 0.6 |
| Neuraminidase | 2.8 | 0.1 | 1.6 |
| Elongation factor 2 | 2.2 | 4.8 | 2.5 |
| F‐box DNA helicase 1 | 1.7 | 4.9 | 0.2 |
| EGF‐containing fibulin‐like extracellular matrix protein 1 | 1.7 | 7.0 | 0.1 |
| Pigment epithelium‐derived factor | 1.4 | 5.1 | 0.4 |
| Procollagen C‐endopeptidase enhancer 1 | 1.4 | 9.4 | 0.1 |
| Heterogeneous nuclear ribonucleoprotein Q | 1.2 | 3.2 | 0.2 |
| Dystroglycan | 1.0 | 6.1 | 0.2 |
| Protein CYR61 | 1.0 | 1.6 | 0.7 |
| Follistatin‐related protein 1 | 0.8 | 2.9 | 0.2 |
| Retinoid‐inducible serine carboxypeptidase | 0.8 | 1.4 | 0.2 |
| Lysosomal protective protein | 0.8 | 5.8 | 0.2 |
| Ιmportin‐5 | 0.8 | 4.3 | 0.1 |
| Nucleobindin‐1 | 0.7 | 1.3 | 0.0 |
| Dipeptidyl peptidase 3 | 0.7 | 2.6 | 0.1 |
| Nucleobindin‐2 | 0.6 | 1.6 | 0.0 |
| Cathepsin B | 0.6 | 1.8 | 0.0 |
| 78 kDa glucose‐regulated protein | 0.6 | 2.7 | 0.0 |
| Nidogen‐1 | 0.5 | 5.7 | 0.0 |
| cAMP‐dependent protein kinase catalytic subunit gamma | 0.5 | 4.7 | 0.0 |
| Legumain | 0.4 | 1.4 | 0.3 |
| 45 kDa calcium‐binding protein | 0.4 | 3.9 | 0.1 |
| Actin‐17 | 0.3 | 1.5 | 0.1 |
| U2 small nuclear ribonucleoprotein A' | 0.3 | 1.2 | 0.1 |
| Actin‐10 | 0.3 | 0.4 | 0.0 |
| Spermidine/putrescine import ATP‐binding protein | 0.3 | 1.0 | 0.0 |
| Putative phospholipase B‐like 2 | 0.2 | 1.2 | 0.0 |
| 40S ribosomal protein S6 | 0.2 | 3.7 | 0.1 |
| Actin, gamma | 0.2 | 1.4 | 0.1 |
| Collagen alpha‐l (VI) chain | 0.2 | 0.9 | 0.0 |
| Bone morphogenetic protein 1 | 0.2 | 0.9 | 0.1 |
| Annexin A5 | 0.2 | 0.3 | 0.0 |
| Rho GDP‐dissociation inhibitor 1 | 0.2 | 2.3 | 0.0 |
| Receptor‐type tyrosine‐protein phosphatase S | 0.2 | 1.5 | 0.0 |
| Nuclear migration protein nudC | 0.2 | 0.3 | 0.0 |
| Heat shock cognate 71 kDa protein | 0.1 | 3.9 | 0.0 |
| 30S ribosomal protein S6 | 0.1 | 0.2 | 0.0 |
| Heat shock 70 kDa protein | 0.1 | 0.9 | 0.0 |
| Small ubiquitin‐related modifier 2 | 0.1 | 1.1 | 0.0 |
| Chaperone protein dnaK2 | 0.1 | 2.4 | 0.0 |
| Vitamin K‐dependent protein S | 0.1 | 0.6 | 0.0 |
| POTE ankyrin domain family member E | 0.1 | 0.0 | 0.0 |
| Transmembrane protein 132A | 0.1 | 0.8 | 0.0 |
| Matrix metalloproteinase‐19 | 0.1 | 1.3 | 0.0 |
| Protein disulfide‐isomerase | 0.1 | 1.0 | 0.0 |
| Cathepsin Z | 0.1 | 0.4 | 0.0 |
| Actin | 7.7 | 0.0 | 0.0 |
| Drebrin | 3.0 | 0.4 | 0.0 |
| Probable dual‐specificity RNA methyltransferase RlmN | 0.9 | 0.0 | 0.3 |
| EGF‐containing fibulin‐like extracellular matrix protein 1 | 0.4 | 0.0 | 0.1 |
List of the 50 most abundant overlapping HCP common to all three cell lines (top) and four unique to GAA HCP (bottom). Values (calculated µg/mL) are ordered by abundance in GAA sample. HCP are considered “overlapping” when present in at least two out of three cell lines. Concentration (µg/mL) is calculated by multiplying normalized abundance value by molecular weight divided by sample volume (10 µL). Normalized abundance value based on BSA reference standard