Table 2.

Construction of human diabodies. Primer combinations and primer sequences

Template	VL amplification		VH amplification
pHenIX human scFv B10	Vκ1 ApaL1	Jκ1 Not1	VH4 Sfi1/Nco1	JH3 for Sal1
pHenIX human scFv H6	Vκ1 ApaL1	Jκ1 Not1	VH6 Sfi1/Nco1	JH2 for Sal1
pHenIX human scFv D12	Vκ1 ApaL1	Jκ1 Not1	VH4 Sfi1/Nco1	JH2 for Sal1
pHenIX scFv mGuy13	mGuy13 ApaL1	mGuy13 Not1	VH4 Sfi1/Nco1	mGuy13 Sal1

Vκ1 ApaLI: 5'-TGAGCACACAGTGCACTCGACATCCAGATGACCCAGTCTCC-3'

Jκ1 Not1: 5'-GAGTCATTCTCGACTTGCGGCCGCACGTTTGATC/TTCCAC/GCTTGGTCCC-3'

V_H4 Sfi1/Nco1: 5'-GTCCTCGCAACTGCGGCCCAGCCGGCCATGGCCCAGGTGCAGCTGCA/ GGAGTCGGG-3'

V_H6 Sfi1/Nco1: 5'-GTCCTCGCAACTGCGGCCCAGCCGGCCATGGCCCAGGTACAGCTGCA/ GCAGTCAGG-3'

J_H3 Sal1: 5'-GAGTCATTCTCGTGTCGACACGGTGACCATTGTCCC-3'

J_H2 Sal1: 5'-GAGTCATTCTCGTGTCGACACAGTGACCAGGGTGCC-3'

mGuy13 ApaL1I:5'-TGAGCACACAGTGCACTCGACATCGAGCTCACTCAGTCTCC-3'

mGuy13 Not1: 5'-TTTTCCTTTTGCGGCCGCCCGTTTTATTTCCAACTTTGT-3'

mGuy13 Sal1: 5'-GAGTCATTCTCGTGTCGACACGGTGACCGTGGTGCCTTGGCCCCAGTAGTCAAAGTAGGT-3'