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. 2017 May 10;6(7):715–724. doi: 10.1016/j.molmet.2017.05.004

Figure 4.

Figure 4

Effect of ADAMTS5 deficiency on in vitro browning of precursor cells from SC WAT. (A) Oil Red O staining at day 8 of adipogenic differentiation of stromal-vascular cells derived from SC AT of wild-type or Adamts5−/− mice. The scale bar represents 200 μm. (B) Quantification of Oil Red O uptake (n = 6). (C) Adamts5 mRNA levels at day 0 and day 8 of differentiation. (D–E) Gene expression of Ucp1 (D) and Cidea (E). Gene expression levels are relative to wild-type cells at day 0, normalized for the housekeeping gene Tbp. Data are means ± SEM of 4–6 determinations. *p < 0.05, **p < 0.01 versus wild-type.