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. 2017 May 4;6(7):640–650. doi: 10.1016/j.molmet.2017.04.009

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Rock inhibitors Fasudil and RKI-1447 induce endoderm differentiation of hESCs. (A) Graphical quantification of FACS plots showing the variability in WNT3A/AA differentiation induced in hESCs compared to the differentiations induced by the treatment of Fasudil and RKI-1447. (B) FACS plots showing the differentiation of hESCs towards FOXA2 and SOX17. The differentiation towards DE is assessed by the analysis of FOXA2-488 and SOX17-647 conjugated antibodies. (C) Graphical representation of FACS data showing the percentage of FOXA2+/SOX17 + cells. (D) qPCR quantification of ROCKi treated cells showing similar expression of FOXA2 and SOX17 compared to WNT3A/AA. (E) Representative stainings of H9 treated with Fasudil, RKI-1447, and WNT3A/AA showing the expression of FOXA2 (red) and SOX17 (green).