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. 2017 May 19;6(7):651–663. doi: 10.1016/j.molmet.2017.05.005

Figure 2.

Figure 2

Expression of mitochondrial-associated genes and mitochondrial DNA content in control and β-Tfb2m−/−mice. (A) qRT-PCR analysis of Tfb2m mRNA in islets from control and β-Tfb2m−/− mice; n = 3 mice. (B) qRT-PCR analysis of Tfam mRNA (left; n = 3 mice/group) and western blot and densitometry analysis of TFAM protein abundance (right; control, n = 6 mice and β-Tfb2m−/−, n = 5 mice) in islets from control and β-Tfb2m−/− mice. (C) qRT-PCR analysis of Atp8, Co1, Cyb, Nd1, and Rnr2 mRNA in islets from control and β-Tfb2m−/− mice, n = 3 mice/group. (D) Western blot and densitometry analysis of NDUFB8, SDHB, UQCRC2, MT-CO1, and ATP5A protein abundance in in islets from control and β-Tfb2m−/− mice; control, n = 6 mice and β-Tfb2m−/−, n = 5 mice. (E) Mitochondrial DNA content in islets from control and β-Tfb2m−/− mice; control, n = 6 mice and β-Tfb2m−/−, n = 9 mice. Representative images for quantitative western blots are shown. All data are mean ± s.e.m. Statistical differences were examined by unpaired Student's t-test. *P < 0.05, **P < 0.01 and ***P < 0.001 versus the corresponding value for control mice.