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. 2017 May 19;6(7):651–663. doi: 10.1016/j.molmet.2017.05.005

Figure 6.

Figure 6

Silencing of TFB2M in insulin-producing cells results in impaired cellular respiration and decreased ATP content. (A) Left – Oxygen consumption rate (OCR) in control and TFB2M-deficient INS-1 832/13 clonal cells following treatment with glucose, oligomycin, rotenone, and carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP). Right – Calculation of basal, glucose-stimulated and oligomycin-stimulated respiration as well as proton leak in control and TFB2M-deficient INS-1 832/13 clonal cells; n = 5 different cell passages/group. (B) ATP content at 2.8 mM glucose and upon stimulation with 16.7 mM glucose in control and TFB2M-deficient INS-1 832/13 clonal cells; n = 9 different cell passages/group. All data are mean ± s.e.m. Statistical differences were examined by unpaired Student's t-test or by a two-way repeated measures ANOVA followed by Tukey's post-hoc test (A – left) *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus the corresponding value for controls.