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. 2005 Jan 25;102(6):1877–1882. doi: 10.1073/pnas.0401179102

Fig. 4.

Fig. 4.

Histone H1 is an efficient DNA-PK substrate. (A) SDS/PAGE analysis of histone H1 and core histones after phosphorylation by DNA-PK. After kinase assays, the histone substrates were purified by using Vivaspin concentrators with 50-kDa cutoff sizes, loaded onto 12% SDS/PAGE, and visualized by Coomassie blue staining. (B) PhosphorImager detection of [γ-32P]ATP by using the gel shown in A.(C) Comparison of DNA-PK-mediated phosphorylation of histone H1 to that of a standard p53-derived control substrate (Promega).