Figure 2.

miR-200c activates the PI3K/Akt pathway via suppressing expression of FOG2. (a) The level of phosphorylation at Akt residue S473 was significantly higher in LX2-200c cells than LX2-nc cells. (b) 25 μM LY-294002 (a PI3K inhibitor) significantly inhibited the expression of phospho-Akt in LX2-200c cells in a time-dependent manner. (c–e) 25 μM LY-294002 significantly inhibited the proliferation and migration of LX2-200c cells. (f) There was the significantly lower levels of FOG2 expression in LX2-200c cells, while no significant change was observed in PI3K expression. ∗ means p < 0.05, ∗∗ means p < 0.01, and ∗∗∗ means p < 0.001.