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. 2017 May 22;6(7):621–630. doi: 10.1016/j.molmet.2017.05.009

Figure 3.

Figure 3

Activation of the ATF6 pathway in skeletal muscle of F2 female rats from HFD-fed grandfathers. A) Protein abundance of ATF6α-p50, CHOP, GR78, GRP94, p-EIF2A, p-IRE1α, p-JNK, unspliced XBP1(u), and spliced XBP1(s) in EDL muscle of F2 rats. Data are represented as fold change (FC) (n = 7–10). B) Representative blots of protein abundance of targets shown in A. C) Heatmap showing downstream targets of ATF6α retrieved from the transcriptome analysis of GpatHF-HF vs GpatCD-HF animals (n = 4–5). D) Heatmap showing the downstream targets of ATF6α found in the core enrichment of the UPR gene set in GpatHF-HF vs GpatCD-HF animals (n = 4–5). Red: downregulation; Blue: upregulation. E) Expression of ATF6α downstream targets in the core enrichment of the UPR gene set retrieved from the transcriptome analysis of F2 rats (n = 4–5). *p ≤ 0.05: GpatHF-HF vs. GpatCD-HF. GpatCD-HF: HFD-fed offspring from grandfathers fed chow diet; GpatHF-HF: HFD-fed offspring from grandfathers fed HFD.